Cultures of human gut bacteria [Bacteroides thetaiotaomicron VPI-5482 NCBI:txid818 | Bacteroides vulgatus ATCC 8482 NCBI:txid435590 | Akkermansia muciniphila ATCC BAA-835 NCBI:txid349741 | Bifidobacterium longum subsp. infantis ATCC 15697 NCBI:txid391904] (Zengler Lab UCSD) on diluted BHI enriched with coffee extract or chemical compounds under anaerobic conditions. 50% MeOH:water extracts analyzed by LC-MS/MS performed in an UltiMate 3000 UPLC system (Thermo Scientific) using a Kinetex 1.7 um C18 reversed phase UHPLC column (50 X 2.1 mm) and Maxis Impact Q-TOF mass spectrometer (Bruker Daltonics) equipped with ESI source. Data was acquired in positive ion mode.