Monoamine brain content of mainland and island populations of Podarcis siculus

In accordance with the permit of the Ministry of Environment and Energy (Class: AP /L612-07 I 18-481 65; No: 517-07-l-l-l-18-4), and the permit of the Ethical committee of the Faculty of Science, University of Zagreb, Croatia (No: 251-58-10617-18-16), animals (10 males from each location) were captured by noosing, and transported to the laboratory in cloth bags. Since the population on islet Pijavica is strictly protected, this was the maximum number of animals we were permitted to collect.

In the laboratory, animals were maintained in individual terraria (40x30cm) with peat as a substrate, fed every two days with crickets (Acheta domestica), that were supplemented with calcium and vitamins, with water available ad libitum. The light cycle was the same as the natural cycle (14:10 h, light: dark). Room temperature varied between 22°C during the night and 30° C during the day, to simulate natural day-night temperature variation. Lizards were allowed to habituate to laboratory conditions for one week before being subjected to behavioral testing. Brain samples were collected two weeks after the last behavioral test (beginning of July 2018).

The experiments were conducted in accordance with the Directive of the European Parliament and of the Council (2010/63/EU) and the Croatian Animal Protection Law (“Narodne Novine”, 102/17 and 32/19). All efforts were made to reduce the number of animals used and to ensure animal welfare.

依据克罗地亚环境与能源部（类别：AP/L612-07 I 18-481 65；编号：517-07-l-l-l-18-4）及克罗地亚萨格勒布大学科学学院伦理委员会许可（编号：251-58-10617-18-16）的规定，对来自各地点的10只雄性动物进行了套索捕捉，并将之装于布袋中运送至实验室。鉴于Pijavica岛上的动物种群受到严格保护，此乃我们获准采集的最大动物数量。在实验室中，动物被安置于独立的玻璃鱼缸（尺寸为40x30厘米）中，以泥炭作为底床，每隔两天以蟋蟀（Acheta domestica）为食，并补充钙质及维生素，提供充足的水源。光照周期与自然光照周期相同（14:10小时，光：暗）。室内温度在夜间保持在22°C，日间保持在30°C，以模拟自然昼夜温差。在行为测试前，允许蜥蜴适应实验室环境一周。在最后一次行为测试后两周（2018年7月初），收集了脑部样本。实验过程遵循欧洲议会和理事会指令（2010/63/EU）及克罗地亚动物保护法（《人民新闻》，102/17和32/19）。我们尽一切努力减少动物使用数量，并确保动物福利。