Assay development.
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https://figshare.com/articles/dataset/_Assay_development_/839508
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资源简介:
A. The structure of PFKPP derived from UbCH7 L1 loop and c-Cbl RING domain complex. B. The structure of KPATK derived from UbcH7 L2 loop and c-Cbl RING domain complex (PDB ID: 1FBV). C. c-Cbl (RING) – Probe 1 peptide binding isotherms: an increasing concentration of protein (5 nmol/L to 11 µmol/L) was titrated into 100 nmol/L probe probe 1. Kd = 1.67±0.09 µmol/L; Δmp = 150 units. D. c-Cbl (RING) – Probe 2 peptide binding isotherms: an increasing concentration of protein (5 nmol/L to 11 µmol/L) was titrated into 100 nmol/L probe 2. Kd = 0.14±0.08 µmol/L; Δmp = 230 units.
创建时间:
2013-10-31



