Investigation of the impact of endothelial Pnpla2 (Atgl) deletion in mice using snRNAseq

To investigate the impact of endothelial Atgl (Pnpla2) deletion and subsequent endothelial lipid droplet accumulation Atgl-floxed (Atglfl/fl) mice were cross-bred with endothelial cell-specific Cdh5 CreERT2 mice to generate mice with an inducible endothelial cell-specific Atgl-knockout. For analysis, 6-8 week old male mice (Atgl fl/fl LFD, ecAtglKO LFD, Atgl fl/fl HFpEF, ecAtglKO HFpEF, n = 4) were used. To develop HFpEF mice were subjected to a hypertensive-obese two–hit HFpEF model. Briefly, mice received either LFD (10 % energy from fat) or HFD (60 % energy from fat) and N[w]-nitro-l-arginine methyl ester (L-NAME, 0.5 g/L, pH 7.4) in drinking water ad libitum for 15 weeks.