[E-MTAB-345] ChIP-Seq of human MCF-7 cells with anti-ERalpha following estrogen treatment

We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. (Note: Raw data files not provided by submitter) Publication Title: Global analysis of ERbeta binding to human breast cancer cell genome Publication Author List: Grober OMV, Mutarelli M, Paris O, Giurato G, De Filippo MR, Cicatiello L, Ravo M, Ferraro L, Nassa G, Tarallo R, Papa MF, Benes V, Alessandro Weisz. ArrayExpress Release Date: 2010-10-05 Person Roles: submitter Person Last Name: Weisz Person First Name: Alessandro Person Mid Initials: Person Email: [e-mail] Person Phone: Person Address: Person Affiliation: Second University of Naples Experimental Design: compound_treatment_design Experimental Design: in_vivo_design Experimental Design: binding_site_identification_design Experimental Design: high_throughput_sequencing_design Experimental Factor Name: COMPOUND Experimental Factor Name: IMMUNOPRECIPITATE Experimental Factor Name: GENOTYPE Experimental Factor Name: DOSE Experimental Factor Type: compound Experimental Factor Type: immunoprecipitate Experimental Factor Type: genotype Experimental Factor Type: dose Quality Control Type: real_time_PCR_quality_control Quality Control Type: technical_replicate