Raw data for comparison of bioinformatics pipelines for Diatom DNA metabarcoding for ecological assessment

This archive contains the raw .fatsq files for 29 samples from water bodies (lakes and rivers) located in Nordic countries (Sweden, Finland, Norway) sequenced on Illumina MiSeq, with the 18S-V4 marker and with the rbcL marker. For both marker two separate datasets are provided, containing the F and R fragments ( R1 and R2). The samples tags and primer sequences are also provided.

The archive also contains the custom curated reference database used for the taxonomic identification using bioinformatics pipeline. For both marker, two files are provided: an .rarl file with the sequences and sequence ID and a .tax file with the taxonomic information associated.