lncRNA and mRNA profiling in radiated mice bone marrow and hypothalamus pretreated with radioprotectants

To further understanding of lncRNA and mRNA expression during radioprotectation, we used microarray analysis to determine the differential expression of lncRNAs, and mRNAs in the bone marrow of mice exposed to high-dose IR with and without pretreatment with three types of radioprotectants: Amifostine, CBLB502, and Nilestriol, as well in the hypothalamus of mice exposed to IR with and without pretreatment with amifostine. Overall, the induction of lncRNAs and mRNAs was increased by pretreatment with all three radioprotectants. Amifostine treatment led to a similar expression pattern of lncRNAs, and mRNAs to that of CBLB502 but not to that of nilestriol. The radioprotectants exhibited mostly opposite effects against IR-induced lncRNAs, and mRNAs in bone marrow, and might induce a potential common mechanism of histone gene downregulation only in the face of IR, mainly via nucleosome assembly and related signaling pathways, which may be a common response to IR-induced biological changes and could explain the radioprotection mechanism. These results expand the biological function of lncRNAs, and mRNAs in mediating the activity of radioprotectants, further offering insights into the molecular mechanism underlying radioprotection. Radiation induced lncRNA and mRNA expression in mice bone marrow and hypothalamus pretreated with amifostime, CBLB502 and nilestriol was measured at 12 hours after exposure to doses of 8 Gy g-rays. The bone marrow from three animals were pooled in one sample for each treatment.