Degradome analysis of heat stress response in wheat at flag leaf and 10 days after anthesis using

To ascertain the regulatory roles of the miRNAs, degradome sequencing of the 8 PARE libraries was done to identify the target genes which resulted in more than 30M raw reads and above 11M reads in each library after filtering. Using degradome sequencing, 296 targets were identified for a total of 61 known miRNAs. The maximum targets were obtained for members of miR1137a f followed by miR1127b-3p and miR1136. Based on the Gene Ontology (GO) and KEGG pathway enrichment analysis, the majority of these miRNAs and their targets were found to be involved in response to plant development and heat stress