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Data for De novo Comparative Transcriptome Analysis of Genes Differentially Expressed in the Scion of Homografted and Heterografted Tomato Seedlings

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Figshare2020-01-21 更新2026-04-08 收录
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https://springernature.figshare.com/articles/Data_for_De_novo_Comparative_Transcriptome_Analysis_of_Genes_Differentially_Expressed_in_the_Scion_of_Homografted_and_Heterografted_Tomato_Seedlings/10075001/1
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This data record contains a single .xml file holding annotation data, analysis results and primer sequences for comparitive transcriptome analysis of homografted and heterografted tomato seedlings. The data tables correspond to supplementary tables 1-4 in the related research publication.<br>The data file can be accessed via MS Excel, or openly available spreadsheet or notepad software.<br>The related study investigates potential molecular mechanisms involved in plant grafting using genetic approaches, in particular underlying mechanisms implicated in the regulation of development and stress responses at the whole-plant level. The investigation employs transcriptome analysis of grafted plants to reveal specific genes involved in the regulation of grafting-induced physiological responses. Transcriptome results are validated using quantitative real-time PCR (qPCR) analysis.<br>The study provides a foundation for the elucidation of the differentially expressed genes (DEGs) in the scion of hetero- and homografted seedlings. Grafting is significant in vegetable production to enhance plant vigour through the avoidance of soil pathogens and the increased tolerance to low temperature, high salinity, and drought.<br>The data files contains the following elements:<br><b>Supplementary Table S1.</b> The DEGs and functional annotation detected between hetero- and homografted tomato seedlings. Including: gene names, fold changes, biotypes and other annotations.<b>Supplementary Table S2. </b>KEGG pathway enrichment analysis detected between hetero- and homografted tomato seedlings. Including: athway name, mapping and gene counts.<br><b>Supplementary Table S3. </b>Familial classification of transcription factors differentially expressed in the scion of heterografted tomato seedlings, Including: Gene name, References sepcies, family and reference gene introduction.<br><b>Supplementary table S4. </b>Quantitative real-time PCR (RT-qPCR) validated primer sequences for selected differentially expressed genes detected between hetero- and homografted tomato seedlings.<br><br>Raw FASTQ files were deposited in the NCBI Sequence Read Archive under the accession number SUB5937299<br>
提供机构:
Wenli Li; Wenying Zhu
创建时间:
2020-01-21
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