Transcriptomic Profiling of Arabidopsis Cation/H+ Exchanger 20 in response to water deficit stress.

This research project aims to explore the transcriptomic changes of knockout and overexpression lines of Arabidopsis Cation/H+ Exchanger 20 in response to a two-week water deficit stress. Plants were grown in short days for three to four weeks, then submitted to a two weeks water deficit (watering withheld completely). Fully expanded rosette leaves were collected and flash frozen for RNA extraction. Total RNA was extracted using a CTAB protocol and samples were DNAse treated before library preparation for sequencing of messenger RNAs.