Chemokinetic response of St-T1b cells or primary hESCs to PDGF-BB, HB-EGF, TCM and trophoblast secretory products identified by proteome profiling.

St-T1b cells (upper panel) or hESC (lower panel), non-decidualized (ND) or decidualized (D), were subjected to Oris migration assay to monitor random motility, in the presence of PDGF-BB, HB-EGF, TCM, PDGF-AA, PLGF-1, or VEGF-165 at the indicated concentrations. Numbers of cells that had migrated into the detection zone after 18 h incubation were determined and normalized to unstimulated controls within each ND or D group. Shown are the means±SEM of n = 3 independent experiments. Results were analyzed by ANOVA and Dunnett test within each group. ***, P<0.001; **, P<0.01 compared to untreated controls (white columns).