File S1 - Archaeal Tuc1/Ncs6 Homolog Required for Wobble Uridine tRNA Thiolation Is Associated with Ubiquitin-Proteasome, Translation, and RNA Processing System Homologs

Supporting Information. File S1 includes: Table S1. Strains, plasmids used in this study; Table S2. Oligonucleotide primers used in this study; Figure S1. Dendrogram analysis of Haloferax volcanii NcsA and homologs of the α hydrolase (ANH) superfamily from archaea, eukaryotes and bacteria; Figure S2. 3D-structural model of Haloferax volcanii NcsA; Figure S3. Organization of ncsA and its targeted deletion on the genome of Haloferax volcanii; Figure S4. Growth of Haloferax volcanii ΔncsA mutant compared to parent strain H26 at optimum growth temperature; Figure S5. Lys204 residue of NcsA is found isopeptide linked to SAMP2; Figure S6. Detection of E1-like UbaA and PAN-A/1 ATPase in NcsA-StrepII pull-down fractions. (PDF)