Preoperative exercise therapy triggers anti-inflammatory trained immunity of Kupffer cells through metabolic reprogramming

Single-cell transcriptomics revealed that transcriptomic profile is shifted towards anti-inflammatory in Kupffer cells after pre-operative exercise therapy in mice. Overall design: To investigate single cell transcriptomics on Kupffer cells, we obtained hepatic CD45+ leukocytes. Four-week exercise or sedentariness were performed, and liver tissue was harvested, followed by cell isolation, FACs sorting, and 10X Genomics Chromium Drop-Seq and library construction. Illumina Next-Seq sequencing of barcoded single nuclei libraries was performed and aligned to a pre-mrna genome with STAR/CellRanger. Gene/barcode matrices were merged and processed with the Seurat R package.