Single cell transcriptome datasets of murine B cell commitment. Single cell transcriptome datasets of murine B cell commitment

To identify the “time-lapse” TF networks during B lineage commitment, we established multipotent progenitors harboring a tamoxifen-inducible form of Id3, an in vitro system where virtually all cells became B cells within 6 days by simply withdrawing 4-OHT. In this study, single cell transcriptomic analysis at pre- and post-commitment was performed using the culture system. In addition, we also performed single cell RNA-seq analysis of B cell precursor populations (LMPP, CLP and pro-B cells) in murine bone marrow. Overall design: Single cell transcriptomic profiles of multipotent and B committed cells in iLS system and BM-derived B cell precursor populations were analyzed using Chromium Single Cell 3' Solution system (10x genomics).