We compared the five different ways of fragmentation available on a tribrid mass spectrometer and optimized their collision energies with regard to optimal sequence coverage of cross-linked peptides.

NIAID Data Ecosystem90

UHPLC HCD-CID Instrument Iterations, CID

质谱参数优化

仪器验证

Systematic variation of the AGC target and CID resolution to determine the effect on total HCD-CID screening output with other instrumental parameters fixed.

Figshare2018-02-25 更新60

Optimization of LTQ-Orbitrap Mass Spectrometer Parameters for the Identification of ADP-Ribosylation Sites

蛋白质修饰位点鉴定

质谱参数优化

ADP-ribosylation of proteins alters their function or provides a scaffold for the recruitment of other proteins, thereby regulating several important cellular processes. Mono- or poly-ADP-ribosylation

NIAID Data Ecosystem40

UHPLC HCD-CID Instrument Iterations, CID

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