Raw data for Figure 2B: Change of surface marker expression of Mesoangioblasts/HIDEMs upon pro-inflammatory stimulation

HIDEMs and mesoangioblasts were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h. Cells were trypsinized and washed, followed by surface staining for HLA-ABC, HLA-DR, CD40, PD-L1 or fluorochrome matched isotype controls and analysis by flow cytometry. Experiments were carried out in duplicates. n=4. Median fluorescence intensities of the markers were examined, and were shown as Mean ± SE.

HIDEMs与中胚层血管生成细胞在20ng/ml的干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）或白细胞介素-1β（IL-1β）刺激下培养24小时。随后，细胞经过胰蛋白酶消化和洗涤，并进行HLA-ABC、HLA-DR、CD40、PD-L1或荧光标记的等型对照物的表面染色，之后通过流式细胞术进行分析。实验重复进行两次，样本量n=4。检测了标记物的中位荧光强度，并以平均值±标准误的形式呈现。