Small genomic insertions form enhancers that misregulate oncogenes

ChIP-Seq data from 102 cell lines were processed to identify insertions relative to the human reference genome. To confirm the ability of a computational pipeline to identify insertions that are actually present in these genomes, we PCR-amplified and deeply sequenced regions where insertions were predicted to exist in MOLT4 T-cell acute lymphoblastic leukemia cells. The current study release makes available sequences of amplicons from a tumor cell genome (MOLT4) where a computational pipeline predicted small insertions from orthogonal data.]]>
We purchased MOLT4 T-cell leukemia cells from ATCC (CRL-1582). We performed H3K27ac ChIP-Seq in these cells. We processed these data through a computational pipeline to predict insertions in the genomes of these cells. To confirm the presence of predicted insertions, we PCR-amplified and sequenced a subset of these predictions.]]>