Targeting CD36-PPARß signaling-mediated metabolic adaptation in intratumoral Tregs primes tumors for PD-1 blockade

Though high frequency of Tregs in tumor obstructs anti-tumor immune responses, systemic depletion of Tregs caused severe autoimmune disease in mice model. Therefore, specifically targeting intratumoral Tregs is direly needed to fight against tumor. Our study provides a novel approach to target intratumoral Tregs to enhance anti-tumor immunotherapy. Overall design: The goals of this study are to use NGS to perform transcriptome profiling (RNA-seq) to find the changes of the global gene expression programs among Tregs from different tissues including spleen, lymph node, and tumor. Comparison is also done between Tregs isolated from WT (FoxP3-YFPCre+/+) tumor and KO (CD36floxed FoxP3-YFPCre+/-). Total RNA was extracted and submitted to RNA-seq