4 samples from Pacific Ocean uncultured phage metagenome. marine sediment metagenome

Anaerobic oil degrading sediment metagenome - anaerobic subsurface sediments containing Monterey Formation oil and hydrocarbon gasses; sample description: Shane Seep; Coal Oil Point hydrocarbon seep field, offshore Goleta, CA. 0.22 micron filtered; CsCl-gradient; DNAse treatment. ANME metagenome - carbonate mound formed by hydrate crystalization and expansion; actively releasing methane from sediments; sample description: 0.22 micron filtered; CsCl-gradient; DNAse treatment, amplified via MDA with phi29 polymerase. Methanogenic sediments metagenome - anoxic sediments from center of Santa Barbara Basin. Basin water exhibits suboxic conditions at sampling depth. Subsurface environment detailed in refs 1&2. Methanogenesis confirmed through intermittent incubation headspace analyses; sample description: 0.22 micron filtered; CsCl-gradient; DNAse treatment. Benthic methanotrophic mats metagenome - sample cultivated ~1 month above active hydrocarbon gas vent; sample description: 0.22 micron filtered; CsCl-gradient; DNAse treatment.