PFR²

The database offers access to planktonic Foraminifera 18S ribosomal DNA sequence library with a curated taxonomy at the morphological and molecular level. These sequences have been obtained through single-cell DNA extraction from specimens, identified taxonomically by characters of their calcite shells. The vast majority of the sequences cover the fragment of the gene between the helices 32 and 50. The aim of PFR² is to provide a comprehensive and high quality free resource to study the ecology and biogeography of planktonic foraminifera as a model system for evolution in the pelagic realm. The first version of PFR² combines sequences available on Genbank with new data generated across several laboratories. The compilation was facilitated through the efforts of the SCOR/IGBP Working Group 138 “Modern planktonic foraminifera and ocean changes”. A strict control of the quality of the sequences has been applied and the taxonomy has been curated to achieve full internal consistency. PFR² can be used to annotate environmental sequences and also to design probes and primers.

本数据库构建了浮游有孔虫（planktonic Foraminifera）的18S核糖体DNA（18S ribosomal DNA）序列库，并在形态学与分子水平上建立了经整理的分类体系。该库中的序列均来自对标本的单细胞DNA提取，并通过其方解石壳体的特征完成分类学鉴定。绝大多数序列覆盖该基因32号螺旋至50号螺旋之间的片段。本数据库（PFR²）旨在提供一套全面且高质量的免费资源，用于以浮游有孔虫为远洋演化模式类群，开展其生态学与生物地理学研究。PFR²的首个版本整合了GenBank中已收录的序列，以及多个实验室新生成的测序数据。本数据集的汇编得益于SCOR/IGBP第138工作组“现代浮游有孔虫与海洋变化”的支持。本库对序列质量实施了严格管控，并对分类体系进行了统一整理，以确保完全的内部一致性。PFR²可用于环境序列的注释，以及探针与引物的设计。

搜集汇总

背景与挑战

背景概述

PFR²是一个提供浮游有孔虫18S核糖体DNA序列库的数据库，其序列经过形态和分子水平分类学整理，旨在为研究浮游有孔虫的生态、生物地理和进化提供全面、高质量的免费资源。该数据库首版包含3322条序列，整合了GenBank现有数据及多个实验室的新数据，并严格质量控制以确保内部一致性，可用于环境序列注释和探针、引物设计。