Effect of depletion of KEAP1 on gene expression after LPS and IFN-γ stimulation of THP-1 derived macrophages

To investigate the function of KEAP1 in the regulation of macrophage pro-inflammatory polarization, we established a KEAP1 deletion cell line using CRISPR Cas9 technology. We then performed gene expression profiling analysis using data obtained from RNA-seq of 10 different samples at two time points. Comparative gene expression profiling analysis of RNA-seq data for macrophages-derived from WT THP-1 cells and KEAP1-deletion THP-1 cells with or without LPS and IFN-γ stimulation.