Distribution of MJ1 is consistent with horizontal transfer.

Notes1. Presence (+) or absence (−) of MJ1 as determined by genome analysis and/or PCR with subsequent sequencing. The numbers in brackets show the number of MJ1 clones among the number of clones sequenced. For species that produced no PCR product with the MJ1 primer, a positive control with ITS primers was performed, which confirmed genomic DNA integrity.2. The absence of MJ1 is confirmed by searching the genome assembly as well as raw trace sequences.3. In these species, we obtained PCR products when amplifying genomic DNA using the MJ1 primer. However, these products were of incorrect size and were shown to be artifacts as none matched MJ1 when we sequenced 6 (for Oc. caspius), 10 (for Ae. albopictus), and 11 (for An. dirus) clones from these PCR products.