Comparison of transcriptional profiles of peripheral blood mononuclear cells from AIDS patients and healthy control group. Comparison of transcriptional profiles of peripheral blood mononuclear cells from AIDS patients and healthy control group

With the purpose of identifying the cascaded miRNA-mRNA regulatory relationships related to infection of HIV in gene level, we extracted mRNA from Peripheral blood mononuclear cells from 50 AIDS patients and 7 uninfected health controls. All AIDS patients in sample were received Antiretroviral Therapy. To further analysis the gene expression pattern in different virus load, we classified high virus loads (HVL, n=14) and low virus loads (LVL, n=16). 2635 and 2826 differentially expressed genes (DEGs) were selected, respectively in LVL and HVL. Functional enrichment analysis indicated DEGs which involved in immune response and apoptosis, played a crucial role in HIV infection. Further, we identified 5 most influential TFs to build miRNA-TF-mRNA regulatory network. Overall design: In this study, we have included 8 healthy controls and 50 AIDS patients. Peripheral blood mononuclear cells (PBMCs) from morning fasting venous blood samples were collected for RNA extraction and were ran on Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version).