Microarray of 3D cultured basal cells co-cultured with M2-like macrophages

To facilitate the research on the interaction between mammary basal cells and M2-like macrophages, we established a co-culture system of mammary basal cells and M2-like macrophages in vitro, and performed microarray of M2-like macrophage co-cultured basal cells comparing with control, for investigating the changes of downstream signals after co-culturing with M2-like macrophages in the basal cells. The mammary basal cells were sorted from adult female mammary glands by FACS sorting, and co-cultured with M2-like macrophages in 3D for 11-14 days, and the co-cultured cells and control cells were harvested for microarray.