Centripetal integration of past events in hippocampal astrocytes regulated by locus coeruleus

Raw data of astrocytic calcium imaging

Example raw data of calcium imaging of astrocytes in hippocampus of behaving mice. The example includes recording segments with spontaneous behavior and behavior-evoked astrocytic activity, as well as recording segments with optogenetic stimulation of the locus coeruleus with opto-evoked responses. The main goal of the dataset is to convey an impression of raw data quality.

Raw calcium imaging data

The raw calcium imaging data are in the folder 2023-05-11. Each subfolder contains data from three simultaneously acquired imaging planes as .tif files. Between recording segments, a short break was made due to limitations of the data acquisition software. Recordings have already been motion-corrected using non-rigid motion correction. Layer 1 was recorded typically around the pyramidal layer or more ventrally; Layer 2 was typically recorded at the pyramidal layer or more dorsally; Layer 3 (analyzed for the manuscript) was recorded in the stratum oriens of CA1. The extracted average activity across planes is saved as a .mat file in each subfolder for convenient inspection. 

Behavior videos

Behavior videos are saved in the main folder as *.avi files. Their sequence corresponds to the calcium imaging subfolders. The behavioral videos are used to extract face/paw motion as well as pupil diameter as described in the associated manuscript.

Recorded behavioral events

Additional behavioral or experimental events are save in *.mat files (to be opened with MATLAB or Python's scipy.io module). These metadata include the locomotion of the animal (time_running and running), the times of sugar rewards (reward_times) and, when applicable, the timing and properties of optogenetic stimulation (opto_times and opto_protocols).

Relative timing of behavioral and calcium data

Calcium imaging data were recorded at a different framerate and with a small temporal offset compared to the behavioral videos and the behaviorally recorded events. This raw data example mainly serves to illustrate raw data quality and therefore does not include scripts to synchronize behavioral to calcium imaging data. If such a synchronization is necessary to judge the data, please get in touch with the corresponding authors ([e-mail]).