variability of isogenic lymphoblastoid cell line

The purpose of the experiments was to better understand the variability of lymphoblastoid cells on genetic, epigenetic, proteomic, and flow cytometric levels. In these experiments, three LCL cell lines derived from a trio (mother, father, and child) together with five isogenic LCL cell lines derived from a single individual were investigated by flow cytometry. Measurement details and results are presented in the published article.
Conclusion:
Based on the performed experiments even isogenic cell lines derived from a single individual has some degree of variability on the investigated parameters. This variability needs to be taken into accont if different LCL cell lines are investigated. FACS Canto II was calibrated daily, using Cytometer Setup and Tracking fluorescent microbeads (Cat No. 641319, Becton Dickinson, San Jose, CA) and Autocomp software as recommended by the manufacturer. Antibodies were titrated to obtain the optimal staining index and to reduce spillover.