Screening for simple sequences repeat markers in Hemileia vastatrix

Hemileia vastatrix is the most important fungal pathogen affecting Coffea arabica and has invaded nearly every coffee-growing region in the world and the causal agent of recurrent disease epidemics. The development of resistant varieties of coffee against H. vastatrix requires fundamental understanding of the biology of the fungus. However, the complete life cycle of H. vastatrix remains unknown and conflicting studies exist as to whether the fungus is capable of sexual reproduction or not. Here we used population genetics of H. vastatrix to determine the reproductive mode of the fungus across most of its current geographic range. The genetic relationships among H. vastatrix isolates were determined using eight simple sequence repeat markers (SSRs) developed for this study. The analyses of the standardized index of association (rbarD = 0.114 - 0.557, p = 0.001), Hardy Weinberg equilibrium (p ≤ 0.05), and clonal richness (0 - 0.19) all strongly support asexual reproduction of H. vastatri...