Kinetic parameters (23±1°C, pH 7.7) of rAgAChE (WT & G119S) and rhAChE, and Km values for the ATCh-hydrolyzing enzyme in An. gambiae homogenate (G3 and Akron).

aEnzyme velocity at saturating ATCh concentrations; 1 unit (U) = 1 μmol ATCh substrate processed per minute (μmol min−1). Protein concentrations were determined using the Thermo Scientific Micro BCA Protein Assay Kit 23235 (see Materials and Methods). bTurnover numbers (kcat) were determined based on Vmax and the calculated molecular mass of the enzyme catalytic subunits (see Materials and Methods). cSpecific activity determined at [ATCh] = 0.50 mM, according to convention.