RNA sequencing of Patient-Derived Xenografts

Goal: Comparison of mRNA expression profiles in patient-derived xenograft primary tumors Methods: RNA sequencing performed at the High-Throughput Genomics and Bioinformatics Analysis Core at the University of Utah. Detailed procedures available in methods and DNA processing pipline sections. Results: Focus of study compared mRNA expression profiles of samples pre and post passaging in vivo. Unsupervised heirarchical clustering revealed samples clustered together appropriately. Conclusions: The PDX maintain the gene expression profiles of the original tumor. Overall design: Six primary tumor samples are included in the analyses. These same six samples were sequenced again after being passed in mice. Replicates are included.