Disentangling the regulatory role of microRNA of milk exosomes in mastitis of dairy cows

The aim of the research was to evaluate the effect of the mastitis in lactating cows on the differential expressed miRNA in milk exosomes. On the basis of somatic cell count and differential somatic cell counts in the milk, 10 cows were healthy (group G), 11 cows were at risk of mastitis (Group Y) and 11 cows with subclinical mastitis (group R). The exosomes were isolated from milk samples using an isoelectric precipitation and ultrcentrifugation and total RNA extracted. After library preparation, RNA was sequenced to 50 nucleotide long single reads and mapped against Btau_5.0.1. The final number miRNA considered for the analysis was 225. The differentially expressed (DE, pInterconnections among miRNA and target genes in relation to KEGG pathways showed that a regulation of relevant genes and pathways involved in the immune functions affect the expression of miRNa in the milk exosome.