Kinetic parameters of the thiosulfate∶cyanide sulfurtransferase activity of purified MoeBR and MoeZR.
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aThe Thiosulfate∶cyanide sulfurtransferase activity of MBP-MoeZR and MBP-MoeBR were measured by the colorimetric assay after Sörbo [21]. KM and kcat values were determined by varying concentrations of sodium thiosulfate (0.5–60 mM) and potassium cyanide (0.1–40 mM) with 0.5–1 µM enzyme.
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2015-12-02
