GNPS Human AKR1C3 Binds Agonists of GPR84 and Participates in an Expanded Polyamine Pathway

In vitro metabolomics with AKR1C3 and HEPG2 Metabolome Files and corresponding conditions: C3only: Enzyme (AKR1C3) only condition with NADPH co-factor added WTmed: this is medium only control containing HEPG2 extract and NADPH. WTmed+C3: this is a no-cofactor control containing enzyme and HEPG2 extract. WTmed+C3+PH: this is all components together with enzyme, HEPG2 extract and NADPH co-factor. Reversed-phase chromatography was performed with a Kinetex (Cat. # 00G-4601-E0) 5 um C18 100 A column (250 by 4.6 mm), using a water:acetonitrile gradient containing 0.1% formic acid at 0.7 mL/min flow rate: 0-30 min, 10% to 100% acetonitrile. Positive mode (qTOF)