Single cell RNA-sequencing of human bronchial epithelial organoids infected by four different betacoronaivurses; HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2 and mock control

We investigated the interactions of four distinct betacoronaviruses; HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2 within human bronchial epithelial (HBE) organoids using single-cell RNA sequencing (scRNA-seq) to comprehensively understand betacoronaviruses cellular tropism and the intricate interplay between these cells and the host's immune defense mechanisms.