Stomatal response to VPD in C4 plants with different biochemical sub-pathways

C4 plants are integral to many ecosystems around the world and are abundantly found in grasslands and savannas in North America, Australia, and Africa, and deserts in Central Asia among other ecosystems in and around Europe (Edwards and Still 2008; Pyankov et al. 2010; Rudov et al. 2020; Wan et al. 2001). Despite making up 20% of the global plant biomass (Ehleringer et al. 1997), our understanding of C4 physiology and biochemistry still has room for improvements. More specifically, the three subtypes of C4 plants (NADP-malic enzyme, NAD-malic enzyme, and PEP carboxykinase) should be better characterized in terms of their biochemistry. To do that, we require a comprehensive characterization of species from across C4 families, not just model C4 species. However, it is often labor-intensive to collect the full gamut of physiological and biochemical data for large numbers of species. Here, I describe a hierarchical Bayesian approach in parametrizing the C4 photosynthesis model that can esti..., To enable a comparative analysis, we compared 6 closely related C4 lineages that each contain an independent origin of NAD-ME and NADP-ME subtypes, for a total of 12 species. 
We analyzed the response of net CO2 assimilation rate (A) to intercellular concentration of CO2 (Ci) and we measured the response of stomatal conductance (gs) to VPD. All gas exchange measurements were conducted with two Li-6400 Portable Photosynthesis Systems (Li-Cor Biosciences, Lincoln, NE, USA) over a course of five months. We conducted measurements at a constant leaf temperature of 30 °C, PPFD of 1800 µmol m-2s-1, and CO2 concentration at ambient level of 400 ppm, unless otherwise noted. 
We used a hierarchical Bayesian approach to model C4 photosynthesis to estimate maximum PEPC activity (Vpmax) and maximum Rubisco activity (Vcmax) from the A/Ci data. The hierarchical Bayesian approach followed methods of Patrick et al. (2009) and allowed us to estimate multiple parameters (namely Vpmax and Vcmax) simultaneo..., , # Data from: Stomatal response to VPD in C4 plants with different biochemical sub-pathways

[https://doi.org/10.5061/dryad.vx0k6djz5](https://doi.org/10.5061/dryad.vx0k6djz5)

This dataset contains a file of net CO2 assimilation rate (photosynthesis rate) vs intercellular CO2 response for twelve C4 species measured using Li-Cor 6400-XT Portable Photosynthesis System. There are five replicates for each species which were grown in a growth chamber. This dataset can be used in the Bayesian model of C4 photosynthesis coded in R using the package rJAGS (included in the files). The C4 photosynthesis process model is taken from von Caemmerer's C4 photosynthesis model. The Bayesian approach allows hierarchical parametrization of key biochemical variables and can account for error in the data, thus making parametrizations more robust. This model allows the parametrization of maximum ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco the main photosynthetic enzyme) activity, maximum PEP car...