DNA-seq of nitrous oxide reducers and bacterial ammonia-oxidizers: layered soil treatment areaas

Lignocellulose-amended layered soil treatment areas (STAs) provide enhanced N removal by stratifying nitrification and denitrification into a top layer that is filled with sand and promotes nitrification, and a bottom layer that contains sand and sawdust, which provides a C source and electron donor for heterotrophic denitrification. We collected soil cores from three Experimental (layered) STAs and associated Control STAs, containing only sand, and sequenced bacterial ammonia monooxygenase (amoA) and nitrous oxide reductase (nosZ) genes using high-throughput MiSeq Illumina sequencing.