scRNA-seq of colon tumors from myeloid miR-34a knockout mice

miR-34aF/F and miR-34aF/F ;LysM_CRE mice were treated with AOM/DSS to induce coloitis associated cancer (CAC). Tumors were isolated and subjected to scRNA-seq Single cells were loaded on the 10x Chromium system and labeled with the 3’ CellPlex kit set A (10x Genomics) according to the manufacturer’s recommendations. The loaded cell numbers for each genotype were 30,000. Barcoded sequencing libraries were prepared with the Chromium Single Cell 3’ v.3.1 Gene Expression Kit (10x Genomics). Paired-end sequencing (2x100bp) was performed on Illumina NovaSeq 6000 (Illumina) targeting 90,000 reads/cell according to the standard workflow.