An ancient fecundability-associated polymorphism creates a new GATA2 binding site in a distal enhancer of HLA-F (Affymetrix data set)

The role of Gata2 in regulating the expression of HLA in human decidual stromal cells Human primary stromal cells from three different patients were grown to approximately 70% confluence and subsequently treated with 100nM medroxyprogesterone acetate, and 1mM 8-bromo-cAMP and transfected with 60nM scrambled, non-targeting (siNT), or GATA2-targeting (siGATA2) siRNA using Lipofectamine RNAiMax lipid per the manufacturer’s instructions. After 72 hours of transfection exposure cells were harvested and RNA isolated using the RNAeasy Mini kit as per manufacterer’s instructions. cRNA labeling was performed according to Agilent Technologies Inc. protocol using the Agilent Quick Amp Labeling Kit following the two-color manufacturer's protocol.