Transcriptome profiling of NUP133 WT and KO podocytes

We generated NUP133 WT and KO podocytes to model human hereditary SRNS in vitro. A subtype of SRNS is caused by monogenetic mutations of the nuclear pore complex including NUP133. Immortalized human podocytes were genome edited applying the CRISPR/Cas9 technique and two independent NUP133 sgRNAs. Two monoclonal cell backgrounds were used to create two matching sets of NUP133 WT and KO cells (WT-1 versus KO-1 and WT-2 versus KO-2). Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis was performed in duplicate for each cell line. NUP133 KO podocytes exhibit vast transcriptional changes compared to WT cells. Overall design: mRNA analysis of two NUP133 WT and two NUP133 KO immortalized human podocyte cell lines (2 replicates; 2x WT-1, 2x WT-2, 2x KO-1, 2x KO-2).