Single cell RNA sequencing reveals heterogeneity and dynamics of mouse early inguinal white adipose tissue (iWAT) during development

In this study, we comprehensively compared the changes in adipose tissue from 1 week (7 d) to 4 weeks (28 d) postnatally. To analyze the different cell groups and how their amount and function vary along with developmental time, we collected iWAT from 7-, 14-, 18-, 21-, and 28-day-old mice and enzymatically disassociated the tissues into single cells and performed single-cell RNA sequencing. To profile cells in an unbiased manner from iWAT considering the apparent alterations in phenotype and gene expression in mouse iWAT during early life, we collected iWAT from 7-, 14-, 18-, 21-, and 28-day-old mice and enzymatically disassociated the tissues into single cells. Stromal vascular cell fractions (SVFs) were separated from mature lipid-containing adipocytes by centrifugation and were subjected to single-cell RNA-seq.