Sprague Dawley (SD) male rats kidney cortex
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Total RNA from the Sprague Dawley (SD) male rats kidney cortexwas extracted using a Trizol reagent kit (Invitrogen, Carlsbad, CA, USA). For quality control, RNA integrity was checked on an Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA). Oligo (dT) beads were used to isolate the poly mRNA from the total RNA. The enriched mRNA was fragmented and reverse transcribed into cDNA using random primers. The constructed library was sequenced using 3135 an Illumina HiSeqTM 2500 (Illumina, San Diego, CA, USA) by Novogene Biotechnology Co. (Tianjin, China). Expression of transcripts was quantified using RNA-Seq by expectation- maximization (RSEM) software. Transcripts with an absolute fold-change greater than 1.5 and false detection rate (FDR) below 0.05 were classified as differentially expressed. The differentially expressed genes (DEGs) were further subjected to KEGG pathways enrichment analysis.
