Single nucleus tanscriptomic analysis of the mouse enteric nervous system

Enteric neurons are present throughout the GI tract where they integrate local and external signals to regulate GI function and are key modulators of multiple GI diseases. Here, we dissected each layer of the enteric nervous system along the GI tract, and used single nucleus sequencing to identify and characterize neuronal populations. Overall design: The myenteric and submucusal layers of the mouse enteric nervous system were dissected and homogenized. A neuron-specific antibody (HuC/D, Abcam, ab237235; Clone EPR19098) was used to enrich the homogenates for neuronal nuclei. Nuclei were sorted and sequenced using 10X Chromium Next GEM Single Cell 3' Reagent Kits v3.1.