Bulk RNA-seq of human macrophages and monocytes from first-trimester and term placentas

To understand how the human macrophages and monocytes (of both fetal and maternal origin) isolated from first-trimester and term placentas differ, we profiled and compared the transcriptomes of cells freshly isolated by fluorescence-activated cell sorting, including fetal Hofbauer cells, placenta-associated maternal macrophages (PAMM), decidual macrophages, maternal and fetal monocytes using the SMART-Seq® v4 PLUS Kit (Takara Bio).