Towards sensitive, streamlined high throughput protein thermal stability analysis for low sample input

This method development aims to enhance and streamline excisting PISA workflows by reducing pipetting steps and digestion method. With this workflow we want to provide a method with higher reproducibility and simplicity. This method combines the excisting OneTip protocol with low cell concentration input on a 96 well format to reach a high kinase inhibitor target screening throughput from treatment to sample injection within 24h.