Bispecific antibodies and CAR T cells targeting a TP53 mutation-associated neoantigen show discordant affinity requirements

Data deposited here are the sequencing data of scFv from the affMat reported in the study below:
Mutation-associated neoantigens (MANAs) are highly cancer-specific targets for immunotherapy when peptides derived from intracellular mutant proteins are presented on the cell surface via HLA molecules. T cell-engaging bispecific antibodies and CAR T cells can target MANAs to eliminate cancer cells via T-cell activation. However, the low antigen density of MANAs on the cell surface can limit therapeutic efficacy. Here, we investigated whether increasing the affinity of the H2 single-chain variable fragment (scFv) targeting the p53 R175H MANA (HMTEVVRHC presented on HLA-A*02:01) improves its therapeutic effect. We identified higher-affinity H2 variants via phage biopanning and a thiocyanate elution method. Increasing bispecific antibody affinity to the low nanomolar range increased cancer cell killing and tumor control in mouse xenograft models without sacrificing antigen specificity. We next..., , # Bispecific antibodies and CAR T cells targeting a TP53 mutation-associated neoantigen show discordant affinity requirements

Dryad DOI: [https://doi.org/10.5061/dryad.vdncjsz83](https://doi.org/10.5061/dryad.vdncjsz83)

This dataset contains the NGS sequencing data used to score the affinity maturation of the H2 single chain variable fragment (scFv) targeting the p53 R175H MANA (HMTEVVRHC presented on HLA-A*02:01).

## Description of the data and file structure

This dataset contains three files as follows:

1. \"DiNapoliSequencingBarcodeKey_20251016Corrected.xlsx\" is an MS Excel workbook with two worksheets. One each for a sequencing run containing the affinity maturation data from two separate experiments. The name of the worksheets corresponds to the names of the run folders and contains a list of which parts of reads to use to cover the scFv altered regions and a list of the barcode indexes corresponding to the affinity maturation samples.
2. \"032020_Sarah_PhageSeq_9822SP-A.zip\" is...,