Genes related to microtubule functions were downregulated in sinhcaf mutant FG follicles and eggs

Transcriptome sequencing (RNA-seq) analysis uncovered that 1238 genes were upregulated, 923 genes were downregulated in sinhcaf mutant FG follicles, compared to wild type FG follicles. Loss of sinhcaf in mature eggs resulted in upregulation of 904 genes, and downregulation of 911 genes. Downregulated genes in sinhcaf mutant FG follicles and eggs were classified by gene ontology (GO) analysis, then the top 30 GO terms with highest -log10P values were screened out, and at least two downregulated genes were included in each biological processes, cellular components or molecular function term. Microtubules and microtubule-based processes were included in the top 30 GO terms. RNA sequencing was performed on five biological replicates for mature egg, four biological replicates for FG follicle of wild type and sinhcaf mutant zebrafish. Each biological replicate was a pool of mature eggs or FG follicles from one female zebrafish.