Transcriptional changes following HIV infection of choroid plexus brain organoids

We employed iPSC-derived choroid plexus brain organoids to explore the impact of HIV infection on microglia, elucidating alterations in the host gene profile within the central nervous system. We conducted single-cell RNA sequencing to elucidate the changes in microglia upon HIV infection, leading to neural cell damage Choroid plexus brain organoids were infected with mock medium or HIV virus. We pooled 8 or more brain organoids and performed 10x single-cell RNA sequencing. To investigate changes in gene expression in microglia upon HIV infection, we enriched these cells using CD11B+ microbeads