Ptip is essential for tooth development via regulating Wnt pathway. Ptip is essential for tooth development via regulating Wnt pathway

Purpose: the goals of this study are to explore the effect of loss of Ptip in Sp7 positive dental progenitor cells on CD45- Ter119- Tie2- dental progenitor cells Methods: CD45- Ter119- Tie2- dental progenitor cells were sorted by FACs in lower incisors from Ptipf/f and Ptipf/f;Sp7-cre mice and performed RNAseq Our finding reveals that Ptip as crucial regulator in tooth homeostasis, functioning to safeguard the Sp7+ progenitor cells epigenome from a breach in lineage confinement that triggers irreversible tooth defects. Conclusions: loss of Ptip unregulate dental development and active Wnt signal pathway Overall design: RNA was extracted from freshly CD45-Ter119-Tie2- progenitor cells sorted by FACS from lower incisors from Ptipf/f and Ptipf/f;Sp7-cre mice