Evaluating Primer Bias in Full-Length 16S Sequencing of Synthetic and Canine Fecal Samples

This dataset investigates how three distinct 16S rRNA primer sets—including a degenerate design—perform when applied to both synthetic microbial communities and real fecal samples (canine). By comparing taxonomic profiles across multiple primer systems, we highlight primer-induced biases at species-level resolution and underscore the importance of primer design in accurate microbiome profiling. The study provides valuable reference data for researchers seeking to optimize 16S rRNA workflows or benchmark new bioinformatics pipelines.