The cellular heterogeneity and dynamics of bladder mucosa under homeostasis and injured conditions

To comprehensively explore the cellular heterogeneity and dynamics of bladder mucosa under homeostasis and injured conditions, we performed droplet-based scRNA-seq using the 10x Genomics Single Cell Solution on samples isolated from injured and normal rabbit bladder. Here the scRNA-seq data demonstrated that urothelial differentiation and regeneration occur in a linear fashion, with a single origin of basal stem/progenitor cell progressively differentiating into intermediate and superficial umbrella cells. And, Basal cell_2 cells expressing TP63 and higher KRT5 as stem/progenitor cell of rabbit bladder urothelium in normal mucosa-injured conditions. Overall design: Samples isolated from injured (Injury_1, Injury_2) and normal (Normal_1, Normal_2) rabbit bladder droplet-based scRNA-seq to explore the cellular heterogeneity and dynamics of bladder mucosa under homeostasis and injured conditions.