Genomic expression over batch growth of mupirocin producing strain Pseudomonas synxantha NCIMB10586. Genomic expression over batch growth of mupirocin producing strain Pseudomonas synxantha NCIMB10586

The Pseudomonas synxantha strain NCIMB10586 produces the antibiotic mupirocin / Pseudomonic acid A from a 75 kb gene cluster; expression of this is regulated through quorum-sensing. We wished to examine expression of the strain during batch growth, in particular of the mupirocin cluster and any other operons regulated in a similar manner. We took samples from three independent cultures at 7 time-points - sampling every 2 hours from 6 to 18 hours.